High-throughput cryogenic spectroscopy for glycan analysis

ABSTRACT

A method for analyzing a plurality of molecules with cryogenic vibrational spectroscopy including the steps of providing a packet of molecules in a ionized form, injecting the packet into an ion mobility section, spatially separating the ions of the packet into subpackets according to their collisional cross section (CCS), recompressing the subpackets, by removing an empty space between them, loading the ions into a cryogenic ion trap by keeping subpackets with different collisional cross section in a respective separate compartment, cooling the ions in collisions with a buffer gas, tagging the ions by attaching a messenger molecule, sending a pulse to the trap to excite vibrations of the cold, trapped, and messenger-tagged ions, and separately ejecting ion subpacket from the trap into an extraction region of a time-of-flight mass spectrometer and measuring the number of remaining messenger-tagged ions and untagged ions for each subpacket.

CROSS-REFERENCE TO RELATED APPLICATIONS

The present application claims priority to the United States provisionalpatent application with the Ser. No. 62/596,162 that was filed on Dec.8, 2017, the contents thereof being herewith incorporated by referencein its entirety.

FIELD OF THE INVENTION

The invention relates to a method, device, and system for molecularanalysis, more particular glycan analysis.

BACKGROUND DISCUSSION State of the Art and Objectives

Motivation—the Importance of Glycan Analysis

The central tenet of molecular biology is that information encoded inDNA is transcribed into RNA, which in turn is translated intoproteins—the molecules responsible for much of the functioning of livingsystems. The precise template-based nature of this information transferallows one to use information from one class of biological molecules tolearn about, and manipulate, another [1]. This tight connection was thedriving force behind the Human Genome Project, which has beenresponsible for a revolution in the study of biology and medicine.

The picture isn't as neat and tidy as it may seem, however. Many, if notmost, proteins are modified after their translation from RNA. One ofmost common post-translations modifications is the attachment of anotherclass of biopolymer, glycans (otherwise known as carbohydrates,oligosaccharides or sugars), to the side-chains of certain amino-acidresidues in proteins. This process, known as glycosylation, can modifythe function, activity, or localization of a protein and hence plays akey role in living systems. Glycans attached to proteins or lipids arepresent at the surface of almost all cells and mediate cell-to-cellrecognition and signaling, for example [2-5]. They largely govern theinteraction of cells with bacteria and viruses and are central to immuneresponse and inflammation [6]. Glycan molecules on the surface red bloodcells define the various blood groups. In one way or another, glycansare implicated in virtually all major human diseases [1, 2, 4, 5].Understanding these fundamental biological processes requires knowingthe structure of the glycan molecules involved.

In addition to these fundamental motivations for analyzing glycanstructure, there are also practical reasons. Biotherapeutics, orbiologics, are drugs cultivated in living cells and used to treat a widevariety of diseases such as cancer, inflammatory and autoimmunediseases, and rare genetic disorders. Approximately half the drugscurrently in the pharmaceutical pipeline are biologics, and theyrepresent the fastest growing segment of the therapeutic drug market.These biological drugs can be produced in different types of cells andunder different conditions, which leads to differences in theglycosylation pattern, which in turn can affect the drug's effectivenessand cytotoxicity [7-13]. Analysis of the glycans attached to theseprotein therapeutics is thus an important part of the approval processby regulatory bodies [13-15].

There are two factors that render the structural analysis of glycansparticularly complex, however. One is that unlike proteins, glycanbiosynthesis is not template-driven—one cannot determine the sequence byreading a code embedded in DNA. It is controlled by the enzymes thatlink individual monosaccharide building blocks together and trim them,and it is influenced by the cellular environment in which a glycan isproduced. One must therefore analyze the glycans themselves to determinetheir structure.

The other complicating factor comes from the intrinsic chemical natureof glycans—in particular, the wide variety of possible isomerisms:

-   (1) Unlike linear sequences of monomers with distinct mass as found    in proteins and DNA, many of the monosaccharide building blocks of    glycans are isomeric, differing only in the stereochemistry of the    asymmetric carbon atoms.-   (2) The glycosidic bond linking monosaccharides involves a    stereogenic carbon, leading to isomeric α and β anomers.-   (3) Glycosidic bonds can have different attachment points, leading    to different regioisomers.-   (4) A single monosaccharide unit can support multiple glycosidic    bonds, leading to the formation of branched structures that are    isomeric with the corresponding linear chains of the same    monosaccharide content.-   (5) The large number of OH groups allow glycans to be functionalized    at different locations, producing a variety of positional isomers.

Because these various types of isomerism are present simultaneously,there is a vast number of possible glycan isomers [16], and many of thepowerful tools used to sequence proteins are not able to distinguish thesubtle differences between them, making their structuralcharacterization substantially more complicated.

Given the importance of glycans in biological systems and the complexityof determining their primary structure, the development of new tools forthe structural characterization of glycans is vital. A 2012 report ofthe National Research Council of the United States National Academy ofSciences entitled: Transforming Glycoscience: A roadmap for the future[5], which analyzed the state of glycan research, recommended that allUS funding agencies make “high-resolution of structure determination ofcarbohydrate structures and complex mixtures” a high priority. Among theroadmap goals of this report is to develop within ten years “the abilityto routinely determine the complete carbohydrate structure of any glycanor polymer repeat sequence including branching, anomeric linkagesbetween glycans, and substituents.” To achieve this goal, the reportcalled for the “development of new structural techniques”, particularlythose that “use completely novel approaches to primary structuredetermination”.

Existing Techniques for Glycan Analysis

Glycans can be analyzed in various forms—attached to proteins, attachedto peptides, or as free oligosaccharides after enzymatic or chemicalrelease. Since our approach seeks to analyze released glycans, weconsider here only those techniques applied to the liberated species.

The inherent complexity of glycan analysis has led to the use of manydifferent experimental approaches [17-19]. Some techniques, such as NMR[20-22] and X-ray crystallography [23], can be extremely valuable forglycan structural analysis, but they are difficult to implement in ahigh-throughput manner and require considerable amounts of sample. Themost commonly used techniques fall into several categories:chromatographic techniques [17, 24-27], electrophoretic techniques[28-30], mass spectrometry [31-36], ion mobility [37-44] and enzymaticdegradation [45]. Because no one method alone can distinguish all thevarious forms of isomerism present in glycans, it is typical, if notnecessary, to combine at least two of them to get the desiredinformation.

One of the most commonly used chromatographic techniques for glycananalysis is hydrophilic interaction liquid chromatography (HILIC) [27,46], which uses a polar stationary phase and a highly organic mobilephase. Because retention is based on the hydrophilic properties of theanalytes, this approach can distinguish many, though not all, of theisomeric forms of glycans. Used as a stand-alone technique, onetypically derivatizes the molecule with a fluorophore and useslaser-induced fluorescence (LIF) to increase the detection sensitivity.As with many other chromatographic techniques, the retention time iscalibrated with respect to a dextran ladder as an external standard, andcomparison to databases can be used to identify the analytes. To confirmthe structural assignments of this approach and to use it forsequencing, it can be combined with exoglycosidase digestion [17, 47,48]. Another way to use HILIC is to combine it with mass spectrometricdetection [46, 49], which obviates the need for derivatization.Moreover, the high organic content of the mobile phase makesparticularly compatible with electrospray ionization. A major drawbackof this approach is the relatively long retention times required foreach analysis.

Mass spectrometry has become a major tool for glycan analysis due to itsspeed and sensitivity [31-36]. Obtaining structural information requiresthe use of tandem MS techniques, and these have employed a variety ofdifferent dissociation methods to generate the cross-ring fragmentsneeded to determine linkage positions and stereochemistry. Whilepermethylation of the free hydroxyl groups prior to fragmentation helpsto reveal the locations of the glycosidic bonds, on its own, MS cannotdistinguish all of the various glycan isomers. Mass spectrometry hasbeen combined with both chromatographic methods [13, 50, 51] andenzymatic degradation techniques [52] to provide more completestructural characterization, but coupling these methods is not alwaysstraightforward and often requires additional steps, which makes itdifficult to do high-throughput screening of glycans. In contrast, ionmobility spectrometry (IMS), which separates gas-phase ions based ontheir average collisional cross section (CCS) on a millisecondtimescale, is easily coupled to MS, and several studies have shown thatthis combination (i.e., IMS-MS) can resolve many of the glycan isomersthat are indistinguishable by MS alone [37-44]. Nevertheless, IMS isblind to many of the subtle structural details that distinguish isomericglycans [39].

One other possibility is to add a spectroscopic dimension to IMS-MS toachieve further isomer discrimination, since spectroscopic fingerprintscan be extremely sensitive to the slightest differences betweenmolecules. In this direction, several groups have successfully combinedIMS-MS with infrared multi-photon dissociation (IRMPD) spectroscopy foridentification of small glycans [53-57], but the room temperature IRMPDspectra are too broad to uniquely identify isomeric disaccharides in amixture. In very recent work, Mucha et al. [58] used a free-electronlaser to obtain spectroscopic fingerprints of oligosaccharides cooled inliquid helium droplets and demonstrated that sufficiently resolvedspectra could indeed distinguish the various types of isomerism, albeitwith an extremely complex experimental set-up that would be impracticalas a broadly used tool. Moreover, the non-linear nature of theirspectroscopic technique would complicate the comparison of data acrossdifferent platforms.

In light of these deficiencies of the state of the art usingspectrometry for molecule analysis, substantially improved methods,devices, and systems for molecule analysis, in particular glycananalysis are strongly desired.

SUMMARY

According to one aspect of the present invention, a method is providedfor analyzing glycans with cryogenic vibrational spectroscopy,preferably comprising the steps of submitting the glycans to an ionizingprocess to obtain ions; drawing the ions into a vacuum; injecting theions into an ion mobility section and grouping them in packets, eitherbefore or after injection; and in the ion mobility section spatiallyseparating the ions of the packet into subpackets according to theircollisional cross section (CCS), and recompressing the subpackets, byremoving an empty space between them by a compression ratio ion mobilityprogramming. The method further preferably comprises loading the ionsinto a cryogenic ion trap by keeping subpackets with differentcollisional cross section in respective separate compartments; coolingthe ions in collisions with a buffer gas; tagging the ions by attachingone or more messenger molecules and obtaining a weakly bound complex;sending a pulse of infrared light down an axis of the trap to excitevibrations of the cold, trapped, and messenger-tagged ions, andseparately ejecting ion subpackets from the trap into an extractionregion of a time-of-flight mass spectrometer and measuring the number ofremaining messenger-tagged ions and untagged ions for the subpacket.

Moreover, according to another aspect of the present invention, a systemfor analyzing a plurality of molecules with cryogenic vibrationalspectroscopy. Preferably, the system includes a structure for losslession manipulations (SLIM) including an ion mobility section for receivinga packet of molecules in a ionized form, the SLIM configured forspatially separating the ions of the packet into subpackets according toa collisional cross section (CCS) of the ions, for keeping subpacketswith different CCS in a respective separate compartment, and forrecompressing the subpackets by removing an empty space between them, acryogenic ion trap for cooling the ions in collision with a buffer gasand for tagging the ions by attaching a messenger molecule, an opticallight source providing an infrared light to the cryogenic ion trap toexcite vibrations of the cold, trapped, and messenger-tagged ions, and atime-of-flight mass spectrometer having an extraction region forseparately receiving ion subpackets from the cryogenic ion trap formeasuring the number of remaining messenger-tagged ions and untaggedions for each subpacket.

In a preferred embodiment, the method further comprises varying thewavelength of the infrared light pulse and repeating the above-mentionedmeasurement to generate an infrared spectrum of each subpacket of ions.

In a further preferred embodiment, the method further comprisesmeasuring an ion drift time, a mass, and an infrared spectrum for eachion subpacket, comparing the data obtained from the measurements toentries of a determined database to identify species of the glycans.

In a further preferred embodiment, the method comprises fragmenting aparent glycan ion and applying the method to simultaneously measure thedrift time, mass, and infrared spectrum of the resulting fragments.

Despite the great promise of high-resolution vibrational spectroscopyfor glycan identification, there is one major drawback with the state ofthe art approach. By adding a spectroscopic dimension to massspectrometry and ion mobility one increases the measurement time, sincethis approach requires scanning the infrared laser frequency across thespectrum, which can take as long as twenty (20) minutes for each speciesto achieve a sufficient signal-to-noise ratio. The total time requiredto measure a complex mixture that might include 20-30 different glycanswould thus hamper its use as an analytical tool.

As described in detail below, according to an aspect of the presentinvention, the designing and constructing an device is provided thatwill overcome this limitation by multiplexing the measurement of mass,CCS (via ion mobility) and cryogenic vibrational fingerprint spectra ofmany species simultaneously, greatly reducing the average measurementtime. Moreover, this approach will eliminate the time-consumingchromatographic separations and chemical derivatization steps often usedin glycan analysis. Therefore, the resulting high-throughput analyticaltool for glycan identification represents a tremendous breakthrough forthe field of glycoscience.

The above and other objects, features and advantages of the presentinvention and the manner of realizing them will become more apparent,and the invention itself will best be understood from a study of thefollowing description with reference to the attached drawings showingsome preferred embodiments of the invention.

BRIEF DESCRIPTION OF THE SEVERAL VIEWS OF THE DRAWINGS

The above and other objects, features and advantages of the presentinvention and the manner of realizing them will become more apparent,and the invention itself will best be understood from a study of thefollowing description with reference to the attached drawings showingsome preferred embodiments of the invention.

FIG. 1 schematically shows fingerprint vibrational spectra andarrival-time distributions for six isomeric disaccharides that differ intheir anomericity with element A vs B; sequence order of element Bversus C; attachment point of element D versus E; and monosaccharidecontent of element E versus F. The two vibrational spectra in element Dcorrespond to two stable conformations that do not interconvert uponannealing. The infrared spectra were obtained by cryogenic,messenger-tagging spectroscopy [59];

FIG. 2 shows a schematic overview of the device for an experimentalapproach;

FIG. 3 shows a schematic of the basic unit of a Structures for LosslessIon Manipulations (SLIM) module. Element A showing the sandwich of twoprinted circuit (PC) boards that form the separation channel; element Bshowing the various traces on the PC boards; elements C and D thetraveling wave potential. Elements B and C were adapted from Reference[61];

FIG. 4 shows a field created by the radio-frequency (RF) electrodes inthe x-y plane;

FIG. 5 shows a section of a SLIM module incorporating turns;

FIG. 6 shows a serpentine SLIM board with the possibility of switchingthe ions to make addition round trips before exiting;

FIG. 7 shows a principle of compression ratio ion mobility programming(CRIMP). Two separate sections of the SLIM board are wired such that onecan control the advancement of the travelling wave independently. Inthis example, at time steps 1-5, the travelling wave advances at thesame rate on both sections of the board. At time steps 6-13, thestuttered traps (ST) don't advance, while the travelling traps (TT)advance at the same constant rate. At time steps 14-18 the ST begin toadvance again at the same rate as the TT. The net result is that ionpackets that were separated in space have now been put into adjacenttraps;

FIG. 8 shows a representation of the method using the CRIMP technique totake mobility-sorted ions and put them into adjacent travelling traps;

FIG. 9 exemplarily shows a ring electrode trap used to confine ionsradially and trap them axially in separate compartments;

FIG. 10 depicts schematic showing ions trapped in separate compartmentsin the axial direction of the cylindrical electrode trap shown in FIG.9;

FIG. 11 shows a planar implementation of the multi-trap;

FIG. 12 provides for a schematic illustrating the sequential read-out ofeach compartment of the multi-trap by injecting them into the extractionregion of a time-of-flight mass spectrometer and pulsing the extractionelectrodes;

FIG. 13 shows a N-linked glycan unit that is attached to the asparaginesidechain of proteins and then trimmed and modified enzymatically, takenfrom Reference [62];

FIG. 14 Cryogenic, vibrational fingerprint spectrum of common corepentasaccharide motif found in all N-glycans;

FIG. 15 depicts N-glycans showing the common core structure. Adaptedfrom Ref. [1]; and

FIG. 16 shows an exemplary SLIM board that incorporates a series of iontraps for collisional dissociation of a parent glycan molecule.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

Glycans, or oligosaccharides, are ubiquitous in biological systems.Because they decorate the surface of cells, they play a key role invirtually all cellular recognition processes and are implicated inalmost every major disease. Despite their importance, thecharacterization of glycan primary structure lags far behind that ofproteins and DNA because of their intrinsic isomeric complexity. Theisomeric nature of the monosaccharide building blocks, thestereochemistry of the glycosidic bond, the possibility of multipleattachment points, and the occurrence of isomeric branched structuresall make glycans difficult to analyze.

Although mass spectrometry (MS) is one of the most sensitive approachesfor glycan analysis, it has difficulty to distinguish all these varioustypes of isomerisms. Ion mobility spectrometry (IMS) combined with MShas demonstrated some ability to identify glycan anomers andregioisomers, but cannot easily distinguish isomeric disaccharides, forexample.

It has recently been recently demonstrated that cryogenic infraredspectroscopy provides unique vibrational fingerprints of glycans thatdistinguishes all the various types of isomerism. When combined withsimultaneous measurements of mass and ion mobility, these fingerprintscan be tabulated in a database and used to identify a given glycan froma mixture. However, adding a spectroscopic dimension to ion mobility andmass measurements requires additional time, which hampers it use as ananalytical tool. To use spectroscopic data for real-world glycananalysis, one must multiplex the measurement process and record thevibrational spectrum of many species simultaneously.

According to one aspect of the present invention, the goal is to providea novel, transformative approach to molecular analysis, moreparticularly glycan structural analysis. As described further below, wehave recently demonstrated that cryogenic, messenger-taggingspectroscopy, when combined with IMS-MS, is a powerful tool todistinguish even the slightest structural difference between glycanisomers [59, 60]. The high level of isomer discrimination of thisapproach comes from the detailed nature of the spectral fingerprintmeasured at low temperatures. When combined with simultaneousmeasurements of mass and ion mobility, these fingerprints can betabulated in a database and used to identify a given glycan, eitherisolated by itself or in a mixture.

According to one aspect of the present invention, an instrument has beendesigned and constructed that combines state-of-the-art ion mobilityseparation, cryogenic ion spectroscopy, and time-of-flight massspectrometry to perform high throughput analysis of glycan primarystructure, for example as a device, a system, and a method.

One goal of the present invention is to be able to take a mixture ofglycans isolated from a natural source and identify all if itsconstituents, including the specification of the monosaccharide content,the linkage positions and stereochemistry, branching patterns, andsubstitution sites of each species. To achieve this goal, a novel,high-throughput method and system has been developed for analyzingglycan primary structure that combines state-of-the-art ion mobilityspectrometry with cryogenic vibrational spectroscopy and time-of-flightmass spectrometry into a single instrument. The measured data, whichwill include the mass, collisional cross section, and cryogenicvibrational spectrum of each species, will provide a unique fingerprintof individual glycans that will be tabulated in a database and used foridentification. High-throughput will be achieved by multiplexing thespectral measurements of all species in a way that preserves informationobtained by ion mobility separation.

The implementation these aspects of the invention, as a method andcorresponding system, represents a tremendous breakthrough forglycoscience, providing in a powerful new tool for fundamental researchin chemical and molecular biology and a practical diagnostic for thepharmaceutical industry.

Methodology

Introduction

Our proposed method for performing high-throughput analysis of glycanprimary structure is based on a series of proof-of-principle experiments[59, 60]. As shown in FIG. 1 for a series of isomeric disaccharides,infrared spectra in the OH stretching region provide a uniquefingerprint of a given glycan that is exquisitely sensitive to slightestdifferences in its structure, even in cases where ion mobility cannotdistinguish them. Differences in anomericity of the glycosidic bond, theattachment point between monosaccharides, the sequence order, or even inthe change in orientation of a single stereogenic center producesdramatic changes in the pattern of vibrational bands that arestraightforward to detect. This should not be surprising. The OH groupsof a glycan molecule have large dipole moments that interact with eachother through dipole-dipole forces. Even weak interactions will causethe vibrational frequencies of the hydroxyl groups to shift, and giventhe high resolution of spectra taken under cryogenic conditions, one caneasily measure these shifts. Changing the orientation of a particularstereogenic center, for example, changes the entire coupled network,reflecting itself in a change in the vibrational fingerprint. Togetherwith a measurement of the glycan mass and its average cross section(i.e., by ion mobility), these vibrational fingerprints provide a uniqueidentifier for a given glycan molecule.

As promising as these proof-of-principle experiments are, there areseveral requirements for making them into a widely accessible analyticaltechnique.

-   -   1. The measurements must be performed rapidly, with        high-throughput, to provide a sufficiently significant advantage        over existing techniques.    -   2. Vibrational spectra of larger glycans must exhibit        sufficiently distinct spectral fingerprints that can serve as a        unique identifier of the molecule.    -   3. One must be able to construct a functional database using        known standards in a reasonable amount of time.    -   4. There must a mechanism to analyze unknown species (i.e.,        those that we do not find in our database) and add them to the        database.    -   5. Given a mixture of isomeric glycan species that are not        resolved by ion mobility separation, one must be able to        decompose the measured spectra into those of its constituents        and from this determine the relative concentrations.    -   6. The measurements need to be robust and insensitive to small        changes in the experimental conditions so that they can be        performed in a properly equipped laboratory.

Details of the method and system are described hereinafter, includingits capabilities with the above-discussed issues in mind.

Overview of the Experimental Approach

While our proof-of-principle experiments demonstrate that cryogenicvibrational spectroscopy provides unique fingerprints of individualglycans, a number of new technologies need to be introduced to make thisapproach into an analytically useful, high-throughput tool for glycananalysis. We first give an overview of our next-generation instrument,shown schematically in FIG. 2, and then provide details on the featuresthat make these new experiments possible.

Ions generated by nano-electrospray will be drawn into vacuum through aheated capillary and enter a series of ion funnels, which focus the ionsbefore injecting a packet of them into the ion mobility section of theinstrument. Rather than use a standard ion-mobility drift-tube as in ourproof-of-principle experiments, we will employ technology developed bySmith and coworkers called Structures for Lossless Ion Manipulations(SLIM) [61, 63-68], which uses printed circuit board electrodes toperform ion mobility separations. The SLIM is also described in U.S.Pat. No. 8,835,839, U.S. Patent Publication No. 2016/0071715, andInternational Patent Publication No. PCT/US2015/048038, these threereferences herewith incorporated by reference in their entirety. Theions will be spatially separated on the SLIM board according to theircollisional cross section (CCS). As explained in more detail below, theywill then enter a section of the board that recompresses the separatedpackets and removes the empty space between them, using a techniquecalled compression ratio ion mobility programming (CRIMP) [69]. While itmay seem counter-intuitive to separate the ions according to their CCSand then recompress them, the reason for this will soon become clear.

After compressing the mobility distribution, all the ions will be loadedinto a cryogenic ion trap in a way that keeps the packets with differentCCS in separate compartments. Once there, they will be cooled incollisions with cold N₂ buffer gas and tagged by attaching an N₂molecule to form a weakly bound complex. A pulse of infrared light froman optical parametric oscillator (OPO) will be sent down the axis of thetrap to excite vibrations of the cold, trapped, messenger-tagged ions.If a molecule in the trap absorbs an infrared photon, energy will berapidly redistributed throughout its vibrational degrees of freedom,warming it up and knocking off the N₂ tag. Each ion packet will then beseparately ejected from the trap and sent into the extraction region ofa time-of-flight mass spectrometer. Pulsing the extraction electrodeswill send both tagged and untagged ions into a TOF drift tube, wheretheir time of flight (and hence their mass) will be measured, and thefraction of ions that have absorbed a photon and lost its tag will bedetermined from the ion signals at the relevant flight times. Theprocess of ejecting a packet of mobility-separated ions from thecryogenic ion trap into the TOF-MS will be repeated rapidly until allthe packets have been analyzed. Because ion extraction from the trap andthe ensuing TOF measurement can be done at a repetition rate of greaterthan 1 kHz, all the ion packets in the trap can be analyzed in the 100ms between pulses of the infrared OPO. The wavenumber of the OPO is thenincremented, and the entire cycle is repeated. In this way, the iondrift time (which can be converted to cross section), the mass, and theinfrared spectrum is measured for each ion packet in our sample. We willthen compare this data to entries in a database to identify the speciesin our sample. If there are isomers that have not been separated bytheir CCS, we will use the spectra in the database to decompose thespectrum of the mixture and determine its principal components.

Next, each part of the process and method is described in detail.

Details of the Experimental Approach

(i) Ion Mobility Separation Using SLIM

The SLIM technology [61, 63-68] is a type of travelling wave ionmobility that uses a “sandwich” of two opposing PC board electrodes, notunlike those we currently use in our cryogenic ion trap [70]. The basicunit of a SLIM module is illustrated in FIG. 3 (adapted from Ref. [61]).Element A of FIG. 3 shows the two PC boards that form the channel inwhich the ions move. In the example shown, each board includes eleven(11) separate tracks, as shown in B of FIG. 3: six of which carryalternate phases of an RF potential and confine the ions in they-direction, and five of which carry the travelling wave that pushesions in the z-direction. On either side of these eleven tracks are DCguard electrodes that confine the ions in the x-direction. As shown in Band C of FIG. 3, the travelling wave (TW) is made by a repeatingsequence of eight DC pads, four of which at any moment are at a highpotential and the other four are at low potential. The wave “travels” bydropping the potential of pad 1 from high to low and raising thepotential of pad 5 from low to high as shown in C of FIG. 3. Because thesequence is repeated, pads on the corresponding units along the trackare wired together. The travelling wave potential is shown in element Dof FIG. 3. FIG. 4 shows the RF potential in the x-y plane, which formschannels in the x-direction through which the ions propagate.

As in all types of travelling wave ion mobility, ions of differentcollisional cross section are separated as they roll over from onepotential well to the next because of different drag forces through thebath gas. The degree of separation depends upon the height andpropagation speed of the travelling wave as well as the length overwhich the ions are propagated.

Smith and coworkers have demonstrated that one can used these SLIMtracks to direct ions around turns [67], as shown in FIG. 5, whichallows one to create serpentine paths that can extend the overallseparation length on a board of moderate size [65]. For example, on aboard measuring 45.9 cm×32.5 cm, a path length of thirteen (13) metershas been achieved [65].

In additions to turns, they have also introduced T-shaped switches thatcan eject ions from a track onto a perpendicular one. Using theseswitches, one can cause ions to traverse a serpentine path multipletimes before switching them out for detection, further extending thepath length and increasing the resolution [67]. We have designed a SLIMboard that allows for such manipulations, shown in FIG. 6, and have usedSIMION to simulate ion trajectories, demonstrating that we can both turnand switch ions.

The use of SLIM technology has several advantages for the proposedexperiments:

-   -   a) Like our planar ion trap, PCB electrodes are easy to        fabricate and assemble. They can also be easily interchanged.    -   b) SLIM uses low-voltage travelling waves, avoiding the need to        maintain a high voltage across the length of a traditional        linear drift tube.    -   c) The ion transmission efficiency of SLIM devices is very high,        and this will increase the sensitivity for low abundant species        and decrease the overall data collection time compared to our        proof-of-principle experiments.    -   d) The long effective path length and resulting high resolution        achievable using SLIM [63-65] will help distinguish similar        glycan structures. This will simplify the use of spectroscopy to        identify isomers in that fewer of them will overlap in the        mobility dimension.    -   e) A SLIM separation device is compact, reducing the overall        size of the instrument. This also makes it easier to cool        compared to a normal ion mobility drift tube, which further        increases the resolution.    -   f) Once ions are separated by their mobility, they can be easily        manipulated in traveling traps in such a way that keeps them        separate.

According to an aspect of the present invention, a high-throughputapproach is provided that enables substantial advantages over the stateof the art. Once ions are separated by their mobility in the SLIMdevice, one can raise the potential of the travelling waves and turnthem into travelling traps, which no longer achieve separation, sincethe ions simply surf on the potential wall without exiting the trap.This allows us to direct the ions wherever we want and at the same timemaintain them in separated packets according to their CCS. Moreover,because the separation process is “digital”, we can keep track of everytrap and determine exactly which ones include ions. As described below,obtaining information from a “pre-scan” in which the arrival timedistribution is measured and which traps include ions is determinedprovides for an aspect to making multiplexed measurements of vibrationalspectra.

(ii) The Compression Ratio Ion Mobility Programming (CRIMP) Technique—anAspect of the Multiplexing

After having measured the mobility of the ions (i.e., their CCS viatheir arrival-time) on a SLIM board, we want to measure theirfingerprint vibrational spectrum and their mass while retaining theinformation we have already obtained on their mobility. Moreover, wewant to be able to do this in a multiplexed manner, so that we measurethe spectrum of molecules of all CCS and all masses in the sameexperiment.

To do this, information from an ion mobility “pre-scan” can be used todetermine which cells include ions. In general, these ions will beseparated in space. For example, if we have an effective path length ofseveral meters on our SLIM separation board, it is entirely conceivablethat ion packets may be spatially separated by as much as a meter. To beable to multiplex a spectroscopic measurement of ions separated sodistant in space, we need a way to bring them together—otherwise thelength of the ion trap would be impractical. The solution to thisproblem is to use the technique called compression ratio ion mobilityprogramming (CRIMP) [69], albeit in a mode not yet demonstrated by theSmith group.

The basic principle of CRIMP is illustrated schematically in FIG. 7.Ions that have been separated by their mobility can have theirseparation locked in by increasing the potential of the travellingwaves, putting them in what are called traveling traps (TT). The CRIMPtechnique allows one to combine any number of adjacent traps at theinterface of a separate section of the SLIM board that has travelingtraps moving at a different speed, which are called stuttered traps(ST). Basically, the ratio of the speed of the traveling traps to theaverage speed of the stuttered traps define a compression ratio, whichin principle is only limited if one reaches the space-charge limit of aparticular trap.

While current implementations of CRIMP use a fixed compression ratio[69], we will implement it slightly differently. We will use knowledgefrom a pre-scan of the ion arrival-time distribution to determine whichcells have ions in them. Based on this pre-scan, the advancement of thestuttered traps will be programed so as to remove all the empty cellsbetween ion packets, as illustrated schematically in FIG. 8. On the leftare ions separated by SLIM, which are being transported in travellingtraps. Some ion packets may be confined to a single trap, although ingeneral, most mobility peaks will be spread out among multiple adjacenttraps, as illustrated for cells 13-14 and 21-23. By knowing which cellswe want to combine, we can program the ST to move in such a way thateliminates all empty cells and stores ions of different mobility inadjacent TT.

It should be emphasized that although we have compressed the arrivaltime distribution in space, we have maintained the separation of ionsaccording to their mobility, and we know the CCS of each ion packet fromour pre-scan. The next step is to load the mobility-sorted ions into ourcryogenic ion trap, cool them, measure their infrared spectrum, and thenmeasure their mass.

(iii) Multiplexed Spectral Measurements and TOF Analysis

Having sorted the glycan molecules by their CCS and put them in adjacenttraveling traps on the SLIM board, we will then transfer them through adifferential pumping stage into high vacuum. The fields used in the SLIMtraveling traps are strong enough to hold them through differentialpumping and carry them to a cryogenic ion trap, which will be segmentedin the axial direction so that it can include separated packets of ionswithout scrambling them. For this reason, we refer to it as a cryogenic“multi-trap”.

The multi-trap can have various different geometries. One implementationcould be a ring-electrode trap [71], which is a cylindrically symmetricRF device with an effective radial field determined by the ring spacing.A schematic of such a device is shown in FIG. 9.

Opposite phases of an RF voltage are applied to alternate rings,confining the ions in the radial direction [71]. Moreover, because onecan control the DC bias on each ring, one can use them as traps in theaxial dimension as shown schematically below in FIG. 10.

In actual operation, one would control the DC levels of each ring of thetrap in FIG. 9 separately rather than tying every second one together,since this would be needed for loading and unloading.

Another implementation of the multi-trap would be to use a planargeometry, as illustrated in FIG. 11 below. In this exemplary case, thetrap includes a sandwich of two printed circuit boards (PCB). A DCpotential would be applied to the outermost stripes to confine the ionsin the x-direction. The RF stripes on each board would have oppositephases of an RF potential, and this would serve to confine the ions inthe y-direction. The z (axial) dimension would provide the separatecompartments by applying different DC voltages on alternating pads inthe center of the board, producing a potential in the axial dimensionthat resembles that of FIG. 10. Once again, in practice, the actual DCpotentials of the center stripe would each be controlled separately tofacilitate loading and unloading.

Having ions of different mobility in separate compartments in the axialdimension of our multi-trap allows to multiplex the spectralmeasurements of all the ions, and this one aspect of to thehigh-throughput nature of the present method and system. Having loadedthe multi-trap with the mobility-separated ions, we pulse in cold N₂buffer gas, which cools the ions and tags them. We then send an infraredpulse from an optical parametric oscillator (OPO) through the trap,simultaneously irradiating all the ions. If a particular tagged ion inthe trap absorbs an infrared photon, the energy will rapidlyredistribute among the vibrational modes and blow off the weakly boundtag molecule (as in our proof-of-principle experiments). We then ejectone packet of mobility-separated ions at a time from the multi-trap andsend it into the extraction region of a reflectron TOF-MS, as shownschematically in FIG. 12.

The extraction electrodes are pulsed to send the ions into the TOF drifttube, where the tagged and untagged ions separate in time and aredetected. The infrared absorption of a particular ion at the OPOwavelength will be given by the fraction of tagged ion signal that isdepleted upon laser excitation. The ejection process is repeated rapidlyuntil all the packets have been analyzed in the same way. Because theprocess of ion extraction and TOF measurement can be done at arepetition rate of greater than 1 kHz, all the ions in the trap can beanalyzed in the 100 ms between pulses of the infrared OPO. Thewavenumber of the OPO is then incremented, and the entire cycle isrepeated.

If there are species that overlap both in their drift time and theirmass, we will separate them based on a decomposition of the vibrationalspectrum, using the spectra in our database to determine the principalcomponents.

As an example, for a 15-20 cm ring electrode trap, we estimate that weshould be able to fit 15-20 different mobility-separated ion packets inthe axial dimension. As there will almost certainly be some isomericspecies with overlapping CCS, the total number of species in the trapthat we analyze may be as many forty (40). Since the spectrum of eachspecies is measured with one scan of the OPO, this reduces the averagemeasurement time considerably—perhaps less than one minute per specieson average.

Once we have obtained the mass, CCS, and infrared spectrum of eachspecies in our sample, we identify them by comparison with a databasethat we will construct. We discuss the details of this process below.

(iv) A Database Approach to the Determination of Glycan PrimaryStructure

A. General Philosophy of Using a Database

The usual procedure for determining ion structure from spectroscopy isto measure a high-resolution vibrational spectrum and then compare itwith those computed for the lowest-energy structures determined byhigh-level quantum chemical calculations [70, 72, 73]. Once asufficiently good match is found, the computed 3D structure is assignedto the molecule. Because we are interested in only the primary (i.e.,covalent) structure of glycans, including all the various isomerisms,our approach is completely different. A database for glycans isestablished including their respective mass, collisional cross section,and vibrational spectrum. Thereafter, an unknown glycan molecule ormixture is identified by measuring these quantities and comparing itwith entries in the database. This means that our determination ofprimary structure does not depend upon high-level quantum chemicalcalculations, which are extremely difficult for glycans including ofmore than a few monosaccharide units.

While our approach differs from the standard approach used by the ionspectroscopy community, database approaches have been central to glycananalysis [74-76]. Information from chromatographic techniques [45, 49,74, 77], mass spectrometry [78], ion mobility [79, 80], andexoglycosidase digestion [48] have all been collected in databases andused in glycan analysis, and we will complement these existing databasesby constructing one based on cryogenic vibrational spectroscopy. Itwould make strategic sense for us to start by targeting glycan moleculesthat have already been fully characterized by other methods andtabulated in databases, which would relieve the need for us to performour own sequence analysis based on exoglycosidase degradation. It willbe preferable that the results are compared and coordinated with theGlycoMob database of glycan collisional cross sections determined fromon mobility [80]. Once we have a full database entry for a particularglycan, we can rapidly identify it in a sample.

Our spectroscopic database will have the important advantage that thevibrational spectrum is an intrinsic property of the molecule that canbe reproducibly measured in different laboratories. As long as one ismeasuring species cooled to sufficiently low temperatures, the linearvibrational spectrum will be the same every time, even under slightlydifferent conditions in different laboratories. The same cannot be saidfor many other types of tabulated data. For example, measuringchromatographic retention time in glycose units (GU) depends on theproperties of the column, and one must calibrate this against a dextranladder each time. Data from tandem mass spectrometry can differdepending upon the fragmentation technique and the amount of energyimparted. Even ion mobility will depend upon the pressure andtemperature of the drift gas, which must be controlled. In contrast, avibrational spectrum, even if performed on tagged species, is aninherent property of the molecule determined by quantum mechanics,making it much easier to compare from one laboratory to another. Theinfrared laser source needs to be calibrated, but this is done using astandard wavemeter.

B. The Construction of the Database

Given all of the possible isomeric structures for a glycan of a givenmass, it might seem hopeless to try to construct a database large enoughto include them all [16]. Fortunately, there are conserved structures,particularly in the case of N-linked glycans, which helps reducemagnitude of the problem. This comes from the way that N-linked glycansare synthesized: a 14-residue unit, shown in FIG. 13, is covalentlyattached to the asparagine side-chain in the endoplasmic reticulum andthen modified enzymatically [81].

Modification of this initial unit is carried out by enzymes that bothtrim it down but also add additional monosaccharides. After theseprocessing steps, all N-glycans regain a basic core structure includingtwo GlcNAc residues and three (3) mannose residues, as shown in FIG. 14.

We have already measured the cryogenic vibrational spectrum of thisbasic core structure, shown in FIG. 14, which exhibits clearly resolvedspectral features that constitute distinct pattern by which it can beidentified. We will proceed to measure spectra of increasingly complexN-linked glycans, such as those shown in FIG. 15. Each time we add a newspecies to the database, we gain the ability to quickly recognize itwhen it appears in a mixture (i.e., using our technique). Thus, byinitially focusing on N-linked glycans that are already characterized byother methods, we can quickly construct a database that will be usefulfor glycan analysis as it progressively expands. Moreover, thehigh-throughput nature of the experimental approach will expediteexpansion of the database.

C. Use of the Database

The application of our experimental technique will result in amulti-dimensional array including values of mass and CCS along with acorresponding vibrational spectrum. For each element of this matrix, wewould first search the database for all other species of the same mass,since this is the simplest way to classify glycans. Among those in thedatabase with the same mass, we would then search for those that alsomatch the CCS. For a given mass and CCS, if there is only one match inthe database, we would then proceed to compare the measured spectrumwith the one tabulated in the database. If this does not match, itsignifies that the species we are observing has not yet been added tothe database. This case will be discussed in Section D below. If thereis more than one entry that has the same mass and CCS, we will thendecompose the spectrum into a mixture of the species in the databasewith the same mass and CCS to obtain their relative concentrations.Because of the multi-dimensional nature of our measurements, we willhave already limited the number of possible species that could give riseto the spectrum, and the higher the resolution of our SLIM-based ionmobility separation stage, the fewer this number will be. Nevertheless,even if there are a significant number of isomeric species withoverlapping CCS, such as the series of disaccharides shown in FIG. 1,the structured nature of the cryogenic spectra provides a high degree ofinformation content that will make such a decomposition possible.

To help us perform rapid and accurate spectral decompositions todetermine the concentrations of glycans in a mixture, data mining anddatabase searching can be employed. Using our proof-of-principle data,they created synthetic spectra of mixtures with added noise anddemonstrated that one can rapidly decompose them and obtain accuraterelative concentrations. This is because one uses the informationcontent from the entire spectral pattern for identification purposes andnot only isolated peaks.

If after attempting a spectral decomposition using the spectra in ourdatabase the algorithm does not converge, this is a sign that one ormore species with the identified mass and CCS has not been added to thedatabase. We discuss this situation below.

D. The Addition of Unknown Compounds to the Database

While we will initially make a big push to enter as many known glycanstructures as possible into our database by running them through ourmachine and measuring their CCS and vibrational spectrum, as we move toanalyzing mixtures of glycans from biological samples, we will certainlyencounter species that have not yet been entered in our database, and weneed a mechanism by which to determine their primary structure and addthem. This will initially be done by combining existing techniques forglycan analysis with our spectroscopic approach. First, we will purifythe sample chromatographically, using hydrophilic interaction liquidchromatography (HILIC), for example. We will then follow the usualsequencing procedure and use exoglycosidase digestion to successivelycleave off monosaccharides, but in this case we will analyze theresulting products using our spectroscopic technique. If after aparticular step in a series of exoglycosidase digests we find that wereduce the unknown species to one that is in our database, then we canstop—together with the particular exoglycosidase(s) that we have used wecan determine the structure of the original unknown species as well asthe intermediates at each step. As our database grows, starting from thecore N-glycan motif shown in FIG. 14, this process will becomeprogressively simpler and faster. Moreover, we only have to go throughthis time-consuming process once for each unknown species. Once a newcompound is added to the database, it can be rapidly identified thebasis of its vibrational spectrum whenever it appears in a sample.

Another method for identifying unknown glycans and adding them to ourdatabase is to use our high-throughput approach for measuringvibrational spectra to identify glycan fragments that we generate bybreaking up an unknown parent glycan through high-energy collisions. Onecan then determine the parent structure (although not always uniquely)from the identified fragments. FIG. 16 shows a SLIM board on which wehave designed a series of ion traps. We can isolate parent ions in thesetraps and then fragment them by high energy collisions. We will thenanalyze the fragments from a particular parent by reinjecting them ontothe separation section of the SLIM board, measuring their mobility,using CRIMP to recombine them and then measuring the vibrationalspectrum of each of them simultaneously using our cryogenic multi-trap.

CONCLUSIONS

The analysis of glycan structure is undeniably one of the most importantchallenges to furthering our understanding of the molecular basis ofliving systems. According to the aspects of the present invention, ahigh-throughput approach is used in the method and the system to glycanidentification will represent a disruptive technology that will enablemajor breakthroughs in the field of glycoscience. It will provide apowerful new tool for fundamental research in chemical and molecularbiology and a practical diagnostic for the pharmaceutical industry. Itis the type of novel approach to glycan structure determination calledfor by the 2012 report of the United States National Academy of Sciences[5].

In sum, according to some aspects of the present invention, a method andsystem is provided for analyzing glycans with cryogenic vibrationalspectroscopy, the method comprising submitting the glycans to anionizing process to obtain ions; drawing the ions into a vacuum;injecting the ions into an ion mobility section and grouping them inpackets, either before or after injection; and in the ion mobilitysection spatially separating the ions of the packet into subpacketsaccording to their collisional cross section (CCS); and recompressingthe subpackets, by removing any empty space between them by means ofcompression ratio ion mobility programming. The method further comprisesloading the ions into a cryogenic ion trap by keeping subpackets withdifferent collisional cross section in respective separate compartments,cooling the ions in collisions with a buffer gas, tagging the ions byattaching one or more messenger molecules and obtaining a weakly boundcomplex, sending a pulse of infrared light down an axis of the trap toexcite vibrations of the cold, trapped, and messenger-tagged ions, andseparately ejecting each ion subpacket from the trap into an extractionregion of a time-of-flight mass spectrometer and measuring the number ofremaining messenger-tagged ions and untagged ions for each subpacket,and this can be done until all the subpackets have been analyzed. Asystem for performing this method has also been described. A furtherembodiment of this method is to fragment a parent glycan ion andapplying the method to the glycan fragments.

While the invention has been disclosed with reference to certainpreferred embodiments, numerous modifications, alterations, and changesto the described embodiments, and equivalents thereof, are possiblewithout departing from the sphere and scope of the invention.Accordingly, it is intended that the invention not be limited to thedescribed embodiments, and be given the broadest reasonableinterpretation in accordance with the language of the appended claims.

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The invention claimed is:
 1. A method for analyzing a plurality ofmolecules with cryogenic vibrational spectroscopy, the methodcomprising: providing a packet of molecules in a ionized form; injectingthe packet into an ion mobility section; spatially separating the ionsof the packet into subpackets according to their collisional crosssection (CCS); recompressing the subpackets, by removing an empty spacebetween them; loading the ions into a cryogenic ion trap by keepingsubpackets with different CSS in a respective separate compartment;cooling the ions in collisions with a buffer gas; tagging the ions byattaching a messenger molecule; sending a pulse of infrared light downan axis of the trap to excite vibrations of the cold, trapped, andmessenger-tagged ions; and separately ejecting ion subpacket from thetrap into an extraction region of a time-of-flight mass spectrometer andmeasuring the number of remaining messenger-tagged ions and untaggedions for each subpacket.
 2. The method of claim 1, wherein the step ofseparately ejecting is performed until all the subpackets have beenanalyzed.
 3. The method of claim 1, further comprising: repeating thesequence of measurements for different wavelengths of the infrared lightpulse; and generating an infrared spectrum for each subpacket of ionsincluding the fraction of tagged ions as a function of the infraredwavelength.
 4. The method of claim 1, further comprising: measuring anion drift time, a mass, and an infrared spectrum for each ion subpacket;and comparing the data obtained from the measurements to entries of adetermined database to identify species of the molecules.
 5. The methodof claim 1, wherein providing a packet of molecules in a ionized formcomprises: submitting the plurality of molecules to an ionising processto obtain ions; and grouping the ions into a packet.
 6. The method ofclaim 1, wherein the infrared light pulse is generated by an opticalparametric oscillator.
 7. The method of claim 1, wherein the infraredlight pulse is generated by fiber laser pumped solid state laser.
 8. Themethod of claim 1, wherein the buffer gas is N₂.
 9. The method of claim1, a messenger molecule of the messenger-tagged ions is N₂.
 10. Themethod of claim 1, wherein the ion mobility section is a SLIM device.11. The method of claim 1, further comprising: isolating one subpacketof molecules in the ion mobility section; and fragmenting the sub packetwhile still in the ion mobility section, thereby obtaining subpacketsincluding the ion fragments of the initially isolated molecules.
 12. Themethod of claim 1, wherein the molecules are selected from a listcomprising glycans, polypeptides, nucleic acids, lipids, primarymetabolites, and secondary metabolites.
 13. A system for analyzing aplurality of molecules with cryogenic vibrational spectroscopy, thesystem comprising: a structure for lossless ion manipulations (SLIM)including an ion mobility section for receiving a packet of molecules ina ionized form, the SLIM configured for spatially separating the ions ofthe packet into subpackets according to a collisional cross section(CCS) of the ions, for keeping subpackets with different CCS in arespective separate compartment, and for recompressing the subpackets byremoving an empty space between them; a cryogenic ion trap for coolingthe ions in collision with a buffer gas and for tagging the ions byattaching a messenger molecule; an optical light source providing aninfrared light to the cryogenic ion trap to excite vibrations of thecold, trapped, and messenger-tagged ions; and a time-of-flight massspectrometer having an extraction region for separately receiving ionsubpackets from the cryogenic ion trap for measuring the number ofremaining messenger-tagged ions and untagged ions for each subpacket.14. The system of claim 13, wherein the optical light source includes anoptical parametric oscillator (OPO) for generating the infrared light.